Description

• Author: Shelley D. Minteer
• Publisher: Humana Press
• Pages: 247

         Over the last ten years, miniaturization has dramatically shrunk the size of both cellular telephones nd chemical instrumentation, and now also holds the promise of developing portable, inexpensive, and isposable analytical systems on a microchip. In Microfluidic Techniques: Reviews and Protocols, handson esearchers review the principles behind successful miniaturization and describe the key techniques for iniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover ot only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and oft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA
analysis, polymerase chain reaction (PCR), immunoassays, and cell reactors. Highlights include PCR on  microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols ollow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment nd reagents, and tips on troubleshooting and avoiding known pitfalls.
         Comprehensive and informative, Microfluidic Techniques: Reviews and Protocols synthesizes the hemistry, biology, and engineering underlying microfluidic devices so that molecular biologists, as well s analytical and clinical chemists, can advance easily from macroscale to microscale biochemical and ioanalytical techniques.

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